Evaluation of Cloning Efficiency Based on the Production of Cloned Diannan Miniature Pigs

Somatic cell nuclear transfer (SCNT) has provided a powerful tool for creating copies of selected individuals of different species since 1997, when the first mammal cloned from adult somatic cells, the sheep “Dolly”, was born [1]. Feasible SCNT procedures have also been established in pigs [2-4]. Although SCNT has also been successfully applied to the cloning of several miniature pigs, including potbelly miniature pigs [5], Clawn miniature pigs [6], Gottingen pigs [7], Yucatan minipigs [8], NIBS strain Evaluation of Cloning Efficiency Based on the Production of Cloned Diannan Miniature Pigs Weirong Pan1#, Guozhong Zhang1#, Yubo Qing1, Honghui Li1, Wenmin Cheng1, Xin Wang1,3, Xiaobing Li1,2, Yingchao Liu1, Yuanyuan Qi1,2, Jia Wang1, Liangxue Lai1, Hong-Ye Zhao4*, Hong-Jiang Wei1,2* 1College of Animal Science and Technology, Yunnan Agricultural University, Kunming, PR China 2Key Laboratory Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201, PR China 3Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 4National Center for Agricultural Biodiversity, Yunnan Agricultural University, Kunming 650201, PR China # These authors contributed equally to this work